|
KMID : 0382619860060020085
|
|
Hanyang Journal of Medicine
1986 Volume.6 No. 2 p.85 ~ p.97
|
|
|
Effect of Gentamicin on the Cytoplasmic Organelles of Epithelial Cells of Renal Proximal Tubules and Renal Glomeruli in Rats
|
|
金載弘/Kim, Jae Hong
徐大圭/Suh, Tae Kyu
|
|
|
Abstract
|
|
|
Gentamicin is a bactericidal antibiotic of the aminoglycoside group. A large variety of infections has been treated successfully with gentamicin. However, long-term use of aminoglycoside antibiotics has been associated with nephrotoxicity which constitute a major drawback in the management of gram-negative bacterial infections.
Aminoglycosides exerts their bactericidal effect by binding to specific protein components of the small ribosomal units resulting in inhibition of protein biosynthesis in bacterial cells. In a similar way, extensive accumulation of aminoglycosides within proximal tubular cells may inhibit protein synthesis, causing alterations of important biochemical regulatory processes.
Gentamicin appear to damage primarily both glomerulus and tubule reversibly or irreversibly depending on various factors such as dose, duration of exposure, age, sex and condition of the nephron at the time of exposure, etc.
Due to the increasing clinical concern with aminoglycoside nephrotoxicity, considerable interest and effort have focused on elucidation of the mechanism of nephrotoxicity of these compounds, but further investigation is needed to achieve complete understanding of the mechanism underlying aminoglycoside nephrotoxicity.
This study was performed to investigate the ultrastructural changes on the cytoplasmic organelles of epithelial cells of renal proximal tubules in rats.
The aminals used were Wistar albino male rats weighing 150 to 200 gm and administered gentamicin sulfate for 7 days, 30 mg per kg of body weight once a day. Rats were sacrificed at 24 hours after 7 days treatment.
The following results were obtained from the electron micrographs:
1. Numerous autophagic vacuoles, several multivesicular bodies, phagosomes, lysosomes and residual bodies are noted in the ectoplasm of tubular epithelial cell. Several pinocytotic vesicles are present near the apical surface of epithelial cell.
2. Golgi complex is atrophied but cisternae of rough endoplasmic reticulum are swollen and ribosomal granules detached from rough endoplasmic reticulum are scarcely seen. Microvilli are well developed. No apparent alterations in mitochondria and nucleus.
3. Basal lamina of glomerular capillary is thickened and contains electron-dense granules.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|